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human cc cell line hela  (ATCC)


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    ATCC human cc cell line hela
    NRF2 signaling inhibition sensitizes CC cells to cisplatin treatment. ( A ) IC50 values of <t>HeLa,</t> <t>SiHa</t> and C33A cells treated with cisplatin for 48 h. The data are representative of 3 biological replicates. ( B ) IC50 values of HeLa, SiHa and C33A cells treated with cisplatin for 48 h. Cells were pretreated with ML385 (1 µM) for 2 hours prior to cisplatin exposure, with ML385 maintained throughout the entire treatment period. The data are representative of 3 biological replicates. ( C ) RT-qPCR showing the mRNA expression of NQO1 in CC cell lines. Cells were pretreated with ML385 (1 µM) for 2 hours prior to cisplatin exposure. The data are representative of 3 biological replicates. ( D ) Representative histograms and gMFI of DCFH-DA in SiHa and HeLa cells. The data are representative of 3 biological replicates. *p< 0.05, **p< 0.01, ***p< 0.001.
    Human Cc Cell Line Hela, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 24158 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+cc+cell+line+hela/HeLa/pmc12554266-25-0-12
    Average 99 stars, based on 24158 article reviews
    human cc cell line hela - by Bioz Stars, 2026-09
    99/100 stars

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    1) Product Images from "Prediction of Cervical Cancer Progression Leveraging HPV16 Integration-Related Genes"

    Article Title: Prediction of Cervical Cancer Progression Leveraging HPV16 Integration-Related Genes

    Journal: International Journal of Women's Health

    doi: 10.2147/IJWH.S543345

    NRF2 signaling inhibition sensitizes CC cells to cisplatin treatment. ( A ) IC50 values of HeLa, SiHa and C33A cells treated with cisplatin for 48 h. The data are representative of 3 biological replicates. ( B ) IC50 values of HeLa, SiHa and C33A cells treated with cisplatin for 48 h. Cells were pretreated with ML385 (1 µM) for 2 hours prior to cisplatin exposure, with ML385 maintained throughout the entire treatment period. The data are representative of 3 biological replicates. ( C ) RT-qPCR showing the mRNA expression of NQO1 in CC cell lines. Cells were pretreated with ML385 (1 µM) for 2 hours prior to cisplatin exposure. The data are representative of 3 biological replicates. ( D ) Representative histograms and gMFI of DCFH-DA in SiHa and HeLa cells. The data are representative of 3 biological replicates. *p< 0.05, **p< 0.01, ***p< 0.001.
    Figure Legend Snippet: NRF2 signaling inhibition sensitizes CC cells to cisplatin treatment. ( A ) IC50 values of HeLa, SiHa and C33A cells treated with cisplatin for 48 h. The data are representative of 3 biological replicates. ( B ) IC50 values of HeLa, SiHa and C33A cells treated with cisplatin for 48 h. Cells were pretreated with ML385 (1 µM) for 2 hours prior to cisplatin exposure, with ML385 maintained throughout the entire treatment period. The data are representative of 3 biological replicates. ( C ) RT-qPCR showing the mRNA expression of NQO1 in CC cell lines. Cells were pretreated with ML385 (1 µM) for 2 hours prior to cisplatin exposure. The data are representative of 3 biological replicates. ( D ) Representative histograms and gMFI of DCFH-DA in SiHa and HeLa cells. The data are representative of 3 biological replicates. *p< 0.05, **p< 0.01, ***p< 0.001.

    Techniques Used: Inhibition, Quantitative RT-PCR, Expressing

    Related Articles

    Control:

    Article Title: The Inhibitory Effect of an HPV16E7-Specific Affibody Conjugated to Granzyme B (GrB) on Cervical Cancer Cells by Bifunctional Activity
    Article Snippet: Plasmids containing coding sequences, namely, pET21a(+)/ZHPV16E7, pET21a(+)/ZHPV16E7-GrB, and pET21a(+)/Zwt-GrB (the wild-type Zwt affibody without affinity screening, as an untargeted control) were constructed and maintained in our laboratory. .. The HPV16-positive human CC cell lines SiHa (ATCC (HTB-35)) and CaSki (ATCC (CRM-CRL-1550)), the HPV18-positive human CC cell line HeLa (ATCC (CCL-2.1), as the HPV type control), and the HPV-negative human CC cell line C33A (ATCC (HTB-31), as the HPV-free cell control) were purchased from the Cell Bank of the Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences (Shanghai, China) and cultured in RPMI-1640 medium supplemented with 100 mg/L penicillin and streptomycin (Gibco, USA) and 10% foetal bovine serum (FBS) at 37°C in a humidified atmosphere of 5% CO2. .. 2.2 Reagents, Antibody, and Animals The following reagents were used: restriction enzymes (BamH I, Hind III, Sma Ι), DNA and protein markers, and 2 × PCR Master Mix (MBI Ferments, Burlington, Ontario, CA); EndoFree Plasmid Maxi Kits, isopropyl β-D-1-thiogalactopyranoside (IPTG), and Ni-NTA agarose (QIAGEN, Hilden, Germany); RPMI-1640 medium, FBS, penicillin, and streptomycin (Gibco, USA); LipofectamineTM 3000 reagent (Thermo, USA); DeadEndTM Fluorometric TUNEL System (Promega, USA); Annexin V-FITC/PI apoptosis kit (MultiSciences, China); LDH Release Assay Kit, CellTiter-LumiTM Luminescent Cell Viability Assay Kit (Beyotime, China); and human interleukin 18 (IL-18), human interleukin 1β (IL-1β), and high mobility group protein B1 (HMGB-1) ELISA kits (Elabscience, China).

    Cell Culture:

    Article Title: The Inhibitory Effect of an HPV16E7-Specific Affibody Conjugated to Granzyme B (GrB) on Cervical Cancer Cells by Bifunctional Activity
    Article Snippet: Plasmids containing coding sequences, namely, pET21a(+)/ZHPV16E7, pET21a(+)/ZHPV16E7-GrB, and pET21a(+)/Zwt-GrB (the wild-type Zwt affibody without affinity screening, as an untargeted control) were constructed and maintained in our laboratory. .. The HPV16-positive human CC cell lines SiHa (ATCC (HTB-35)) and CaSki (ATCC (CRM-CRL-1550)), the HPV18-positive human CC cell line HeLa (ATCC (CCL-2.1), as the HPV type control), and the HPV-negative human CC cell line C33A (ATCC (HTB-31), as the HPV-free cell control) were purchased from the Cell Bank of the Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences (Shanghai, China) and cultured in RPMI-1640 medium supplemented with 100 mg/L penicillin and streptomycin (Gibco, USA) and 10% foetal bovine serum (FBS) at 37°C in a humidified atmosphere of 5% CO2. .. 2.2 Reagents, Antibody, and Animals The following reagents were used: restriction enzymes (BamH I, Hind III, Sma Ι), DNA and protein markers, and 2 × PCR Master Mix (MBI Ferments, Burlington, Ontario, CA); EndoFree Plasmid Maxi Kits, isopropyl β-D-1-thiogalactopyranoside (IPTG), and Ni-NTA agarose (QIAGEN, Hilden, Germany); RPMI-1640 medium, FBS, penicillin, and streptomycin (Gibco, USA); LipofectamineTM 3000 reagent (Thermo, USA); DeadEndTM Fluorometric TUNEL System (Promega, USA); Annexin V-FITC/PI apoptosis kit (MultiSciences, China); LDH Release Assay Kit, CellTiter-LumiTM Luminescent Cell Viability Assay Kit (Beyotime, China); and human interleukin 18 (IL-18), human interleukin 1β (IL-1β), and high mobility group protein B1 (HMGB-1) ELISA kits (Elabscience, China).



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    Matrix stiffness promotes migration and invasion of CC cells and activates Piezo1. A Microscopy showing typical morphologies of <t>HeLa</t> <t>and</t> <t>SiHa</t> cells cultured on soft and stiff substrates. Scale bar: 100 μm; B Representative immunofluorescence images of HeLa and SiHa cells cultured on soft and stiff substrates in detecting phalloidin (green) and DAPI(blue). Scale bar: 50 μm; C Western blot results showed the expression of Piezo1 in HeLa and SiHa cells cultured on soft and stiff substrates. GAPDH was used as a control; D Quantization of C ( E , G ) Cell migration and invasion assays were performed using HeLa and SiHa cells cultured on soft and stiff substrates. F , H Quantization of E and G ; * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001
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    Image Search Results


    NRF2 signaling inhibition sensitizes CC cells to cisplatin treatment. ( A ) IC50 values of HeLa, SiHa and C33A cells treated with cisplatin for 48 h. The data are representative of 3 biological replicates. ( B ) IC50 values of HeLa, SiHa and C33A cells treated with cisplatin for 48 h. Cells were pretreated with ML385 (1 µM) for 2 hours prior to cisplatin exposure, with ML385 maintained throughout the entire treatment period. The data are representative of 3 biological replicates. ( C ) RT-qPCR showing the mRNA expression of NQO1 in CC cell lines. Cells were pretreated with ML385 (1 µM) for 2 hours prior to cisplatin exposure. The data are representative of 3 biological replicates. ( D ) Representative histograms and gMFI of DCFH-DA in SiHa and HeLa cells. The data are representative of 3 biological replicates. *p< 0.05, **p< 0.01, ***p< 0.001.

    Journal: International Journal of Women's Health

    Article Title: Prediction of Cervical Cancer Progression Leveraging HPV16 Integration-Related Genes

    doi: 10.2147/IJWH.S543345

    Figure Lengend Snippet: NRF2 signaling inhibition sensitizes CC cells to cisplatin treatment. ( A ) IC50 values of HeLa, SiHa and C33A cells treated with cisplatin for 48 h. The data are representative of 3 biological replicates. ( B ) IC50 values of HeLa, SiHa and C33A cells treated with cisplatin for 48 h. Cells were pretreated with ML385 (1 µM) for 2 hours prior to cisplatin exposure, with ML385 maintained throughout the entire treatment period. The data are representative of 3 biological replicates. ( C ) RT-qPCR showing the mRNA expression of NQO1 in CC cell lines. Cells were pretreated with ML385 (1 µM) for 2 hours prior to cisplatin exposure. The data are representative of 3 biological replicates. ( D ) Representative histograms and gMFI of DCFH-DA in SiHa and HeLa cells. The data are representative of 3 biological replicates. *p< 0.05, **p< 0.01, ***p< 0.001.

    Article Snippet: Human CC cell line HeLa, SiHa and C33A were obtained from the American Type Culture Collection (ATCC).

    Techniques: Inhibition, Quantitative RT-PCR, Expressing

    Matrix stiffness promotes migration and invasion of CC cells and activates Piezo1. A Microscopy showing typical morphologies of HeLa and SiHa cells cultured on soft and stiff substrates. Scale bar: 100 μm; B Representative immunofluorescence images of HeLa and SiHa cells cultured on soft and stiff substrates in detecting phalloidin (green) and DAPI(blue). Scale bar: 50 μm; C Western blot results showed the expression of Piezo1 in HeLa and SiHa cells cultured on soft and stiff substrates. GAPDH was used as a control; D Quantization of C ( E , G ) Cell migration and invasion assays were performed using HeLa and SiHa cells cultured on soft and stiff substrates. F , H Quantization of E and G ; * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001

    Journal: Discover Oncology

    Article Title: The increased matrix stiffness caused by LOXL2 activates Piezo1 channels to promote the migration and invasion of cervical cancer cells

    doi: 10.1007/s12672-025-02456-9

    Figure Lengend Snippet: Matrix stiffness promotes migration and invasion of CC cells and activates Piezo1. A Microscopy showing typical morphologies of HeLa and SiHa cells cultured on soft and stiff substrates. Scale bar: 100 μm; B Representative immunofluorescence images of HeLa and SiHa cells cultured on soft and stiff substrates in detecting phalloidin (green) and DAPI(blue). Scale bar: 50 μm; C Western blot results showed the expression of Piezo1 in HeLa and SiHa cells cultured on soft and stiff substrates. GAPDH was used as a control; D Quantization of C ( E , G ) Cell migration and invasion assays were performed using HeLa and SiHa cells cultured on soft and stiff substrates. F , H Quantization of E and G ; * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001

    Article Snippet: Human CC cell lines HeLa and SiHa were obtained from the American Type Culture Collection (ATCC) and were cultured at 37 °C in a humidified atmosphere of 5% CO2 in high-glucose Dulbecco’s modified Eagle’s medium (DMEM) containing 10% FBS with 100 U/ml penicillin and 100 U/ml streptomycin.

    Techniques: Migration, Microscopy, Cell Culture, Immunofluorescence, Western Blot, Expressing, Control

    Knocking down of Piezo1 suppressed the migration and invasion of CC cells. A – C Microscopy imaging showed that the transfection was successful; D – F Western blot analysis of Piezo1 expression in HeLa and SiHa cells after Piezo1 knockdown; G , H Cell migration detected via wound healing assay after Piezo1 knockdown; I , J Quantization of G and H ; K , L Cell migration and invasion assays were examined by Transwell after Piezo1 knockdown; M , N Quantization of K and L ; * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001

    Journal: Discover Oncology

    Article Title: The increased matrix stiffness caused by LOXL2 activates Piezo1 channels to promote the migration and invasion of cervical cancer cells

    doi: 10.1007/s12672-025-02456-9

    Figure Lengend Snippet: Knocking down of Piezo1 suppressed the migration and invasion of CC cells. A – C Microscopy imaging showed that the transfection was successful; D – F Western blot analysis of Piezo1 expression in HeLa and SiHa cells after Piezo1 knockdown; G , H Cell migration detected via wound healing assay after Piezo1 knockdown; I , J Quantization of G and H ; K , L Cell migration and invasion assays were examined by Transwell after Piezo1 knockdown; M , N Quantization of K and L ; * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001

    Article Snippet: Human CC cell lines HeLa and SiHa were obtained from the American Type Culture Collection (ATCC) and were cultured at 37 °C in a humidified atmosphere of 5% CO2 in high-glucose Dulbecco’s modified Eagle’s medium (DMEM) containing 10% FBS with 100 U/ml penicillin and 100 U/ml streptomycin.

    Techniques: Migration, Microscopy, Imaging, Transfection, Western Blot, Expressing, Knockdown, Wound Healing Assay

    ECM stiffness modulated CC progression through Piezo1. A , B Cell migration and invasion assays were performed using HeLa cells cultured on soft and stiff substrates after Piezo1 knockdown; C , D Quantization of A and B ; E , F Cell migration and invasion assays were performed using SiHa cells cultured on soft and stiff substrates after Piezo1 knockdown; G , H Quantization of E and F ; * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001

    Journal: Discover Oncology

    Article Title: The increased matrix stiffness caused by LOXL2 activates Piezo1 channels to promote the migration and invasion of cervical cancer cells

    doi: 10.1007/s12672-025-02456-9

    Figure Lengend Snippet: ECM stiffness modulated CC progression through Piezo1. A , B Cell migration and invasion assays were performed using HeLa cells cultured on soft and stiff substrates after Piezo1 knockdown; C , D Quantization of A and B ; E , F Cell migration and invasion assays were performed using SiHa cells cultured on soft and stiff substrates after Piezo1 knockdown; G , H Quantization of E and F ; * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001

    Article Snippet: Human CC cell lines HeLa and SiHa were obtained from the American Type Culture Collection (ATCC) and were cultured at 37 °C in a humidified atmosphere of 5% CO2 in high-glucose Dulbecco’s modified Eagle’s medium (DMEM) containing 10% FBS with 100 U/ml penicillin and 100 U/ml streptomycin.

    Techniques: Migration, Cell Culture, Knockdown